イマシロ　チカヒロ   Imashiro Chikahiro   今城　哉裕    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.
掲載誌名	正式名：Biotechnology and bioengineering 略　 称：Biotechnol Bioeng ISSNコード：10970290/00063592
掲載区分	国外
巻・号・頁	118(10),pp.3760-3769
著者・共著者	IMASHIRO Chikahiro†, AZUMA Tetsuya, ITAI Shun, KURIBARA Taiki, TOTANI Kiichiro, ONOE Hiroaki, TAKEMURA Kenjiro*
担当区分	筆頭著者
発行年月	2021/06
概要	To generate three-dimensional tissue in vitro, promoting vasculogenesis in cell aggregates is an important factor. Here, we found that ultrasound promoted vasculogenesis of human umbilical vein endothelial cells (HUVECs). Promotion of HUVEC network formation and lumen formation were observed using our method. In addition to morphological evaluations, protein expression was quantified by western blot assays. As a result, expression of proteins related to vasculogenesis and the response to mechanical stress on cells was enhanced by exposure to ultrasound. Although several previous studies have shown that ultrasound may promote vasculogenesis, the effect of ultrasound was unclear because of unregulated ultrasound, the complex culture environment, or two-dimensional-cultured HUVECs that cannot form a lumen structure. In this study, regulated ultrasound was propagated on three-dimensional-monocultured HUVECs, which clarified the effect of ultrasound on vasculogenesis. We believe this finding may be an innovation in the tissue engineering field. This article is protected by copyright. All rights reserved.
DOI	10.1002/bit.27852
PMID	34110012