タナベ　ケンジ   TANABE Kenji   田邊　賢司    所属   研究施設 研究施設    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読なし
表題	Image-based phenotypic profiling of a chemogenomic screening library identifies novel druggable targets in the EGFR pathway
掲載誌名	正式名：BioRxiv
掲載区分	国外
巻・号・頁	pp.440090
著者・共著者	Kenji Tanabe
担当区分	筆頭著者,責任著者
発行年月	2021/04/17
概要	The gene encoding epidermal growth factor receptor (EGFR) is a major driver gene in cancer. Many drugs targeting EGFR-associated molecules have been developed, yet many have failed in clinical trials due to a lack of efficacy and/or unexpected side effects. In this study, I used image-based phenotypic profiling to screen a pharmacologically active compound library with the aim of identifying new druggable targets in the EGFR pathway. As anticipated, the phenotypic screen identified compounds that produce phenotypes resulting from targeting a known specific molecule or pathway. The assay also showed that compounds with diverse known mechanisms of action produced similar, EGFR-related cellular phenotypes. Biochemical assays revealed that those compounds share a previously unappreciated common target/pathway, showing that the image-based assay can identify new target molecules that are independent of the compound's known target. Further experiments showed that ROCK1 and PSMD2 are novel druggable targets within the EGFR pathway.
DOI	https://doi.org/10.1101/2021.04.16.440090