オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	総説
言語種別	英語
査読の有無	査読なし
表題	Cell sheet tissue engineering for scaffold-free three-dimensional (3D) tissue reconstruction.
掲載誌名	正式名：Methods in cell biology 略　 称：Methods Cell Biol ISSNコード：0091679X/0091679X
掲載区分	国外
巻・号・頁	157,pp.143-167
著者・共著者	KIM Kyungsook†*, BOU-GHANNAM Sophia, OKANO Teruo*
担当区分	最終著者,責任著者
発行年月	2020/01/21
概要	Three-dimensional (3D) reconstruction of highly functional tissues is of great importance in advancing the clinical benefit of tissue engineering and regenerative medicine. In the last quarter century, many studies have found that by engineering a 3D microenvironment that resembles the in vivo tissue condition, cells exhibit behaviors and functions that reflect those of native tissue. Biomaterial scaffolds are a central technology for providing 3D microenvironments in vitro, and, in conjunction with diverse design and cell seeding advents, have produced highly functional and complex 3D tissues. Here, we describe a new approach to creating 3D cell-dense tissue-like constructs without a biomaterial scaffold. Cell sheet technology with cell sheet layering strategies generates highly cell dense, engineered tissue capable of direct crosstalk with the tissue-engraftment surface, in addition to paracrine-mediated signaling. In this chapter, we will introduce methods of reconstructing 3D tissue using cell sheet technology and the advantages of a scaffold-free design.
DOI	10.1016/bs.mcb.2019.11.020
PMID	32334713