ノグチ　タケハル   Noguchi Takeharu   野口　岳春    所属   医学部 医学科（東京女子医科大学病院）    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Rapid Flow Cytometry of Gastrointestinal Stromal Tumours Closely Matches the Modified Fletcher Classification.
掲載誌名	正式名：Anticancer research 略　 称：Anticancer Res ISSNコード：17917530/02507005
掲載区分	国外
巻・号・頁	41(1),pp.131-136
著者・共著者	TANIGUCHI Kiyoaki†, SUZUKI Akane, SERIZAWA Akiko, KOTAKE Sho, ITO Shunichi, SUZUKI Kazuomi, YAMADA Takuji, NOGUCHI Takeharu, AMANO Kunihiko, OTA Masaho, MURAGAKI Yoshihiro, YAMAMOTO Masakazu
発行年月	2021/01/10
概要	AIM:We aimed to develop a rapid, simple procedure and an algorithm for quantitative analysis and classification of the metastatic risk of gastrointestinal stromal tumours (GIST) for clinical use.MATERIALS AND METHODS:Eighteen specimens from laparoscopic local gastrectomy were assessed by flow cytometry. We devised a new risk classification for GIST by combining flow cytometry parameters with tumour size and evaluated whether the combined parameters correlated with the modified Fletcher risk classification.RESULTS:We found a significant correlation between clinical prognostic factors (mitotic count and Ki-67 labelling index) and the flow cytometry parameters DNA ploidy, DNA index and S-phase fraction. The combined parameters established from tumour size and the flow cytometry parameters showed a high correlation with the modified Fletcher risk classification (p=0.0064). Flow cytometry had to be performed for approximately 10 minutes to determine the metastatic risk.CONCLUSION:Rapid flow cytometry parameters can classify risk without the need for histological analysis.
DOI	10.21873/anticanres.14758
PMID	33419806