ナカヤマ　マサミチ   Nakayama Masamichi   中山　正道    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Micropatterned Smart Culture Surfaces via Multi-Step Physical Coating of Functional Block Copolymers for Harvesting Cell Sheets with Controlled Sizes and Shapes.
掲載誌名	正式名：Macromolecular Bioscience 略　 称：Macromol Biosci ISSNコード：16165195/16165187
掲載区分	国外
出版社	Wiley Online Library
巻・号・頁	21,pp.e2000330
著者・共著者	NAKAYAMA Masamichi†*, TOYOSHIMA Yuki, KIKUCHI Akihiko, OKANO Teruo
担当区分	筆頭著者,責任著者
発行年月	2020/12
概要	Cell micropatterning on micropatterned thermoresponsive polymer-based culture surfaces facilitates the creation of on-demand and functional cell sheets. However, the fabrication of micropatterned surfaces generally includes complicated procedures with multi-step chemical reactions. To overcome this issue, this study proposes a facile preparation of micropatterned thermoresponsive surfaces via a two-step physical coating of two different diblock copolymers. Both copolymers contain poly(butyl methacrylate) blocks as hydrophobic anchors for water-stable polymer deposition. At first, thermoresponsive polymer layers are constructed on cell culture dishes via spin-coating block copolymers containing poly(N-isopropylacrylamide) blocks that exhibit a transition temperature of ≈30 °C in aqueous media. To create polymer micropatterns on the thermoresponsive surfaces, microcontact printing of block copolymers containing hydrophilic poly(N-acryloylmorpholine) (PNAM) blocks is performed using polydimethylsiloxane stamps. Stamped PNAM-based block polymers are adsorbed to the outermost thermoresponsive surfaces, and increase the surface hydrophilicity with decreasing protein adsorption. Cells adhere and proliferate on the thermoresponsive domains at 37 °C, whereas the stamped hydrophilic domains remain cell-repellent for 7 days. At 20 °C, cell sheets with controlled sizes and shapes are harvested from the surfaces with the desired micropatterns. This technique is useful for the preparation of micropatterned polymer surfaces for various biomedical applications.
DOI	10.1002/mabi.202000330
PMID	33369185