オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	総説
言語種別	英語
査読の有無	査読あり
表題	Capillary Networks for Bio-Artificial Three-Dimensional Tissues Fabricated Using Cell Sheet Based Tissue Engineering
掲載誌名	正式名：International journal of molecular sciences. 略　 称：Int J Mol Sci ISSNコード：14220067
掲載区分	国外
巻・号・頁	22(1),pp.92
著者・共著者	SEKINE Hidekazu†*, OKANO Teruo
担当区分	最終著者
発行年月	2020/12
概要	Abstract One of the most important challenges facing researchers in the field of regenerative medicine is to develop methods to introduce vascular networks into bioengineered tissues. Although cell scaffolds that slowly release angiogenic factors can promote post-transplantation angiogenesis, they cannot be used to construct thick tissues because of the time required for sufficient vascular network formation. Recently, the co-culture of graft tissue with vascular cells before transplantation has attracted attention as a way of promoting capillary angiogenesis. Although the co-cultured vascular cells can directly contribute to blood vessel formation within the tissue, a key objective that needs to be met is the construction of a continuous circulatory structure. Previously described strategies to reconstruct blood vessels include the culture of endothelial cells in a scaffold that contains microchannels or within the original vascular framework after decellularization of an entire organ. The technique, as developed by authors, involves the progressive stacking of three-layered cell sheets onto a vascular bed to induce the formation of a capillary network within the cell sheets. This approach enables the construction of thick, functional tissue of high cell density that can be transplanted by anastomosing its artery and vein (provided by the vascular bed) with host blood vessels. View Full-Text Keywords: regenerative medicine; tissue engineering; cell sheet technology; vascularization; vascular bed; bioreactor; tissue culture
DOI	10.3390/ijms22010092