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ササキ ダイスケ
Sasaki Daisuke
佐々木 大輔 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 特任助教 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Measuring the contractile force of multilayered human cardiac cell sheets. |
| 掲載誌名 | 正式名：Tissue engineering. Part C, Methods. 略 称：Tissue Eng Part C Methods ISSNコード：19373384/19373392 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 26(9),pp.485-492 |
| 著者・共著者 | SAKAGUCHI Katsuhisa†, TAKAHASHI Hiroaki, TOBE Yusuke, SASAKI Daisuke, MATSUURA Katsuhisa, IWASAKI Kiyotaka, SHIMIZU Tatsuya*, UMEZU Mitsuo |
| 発行年月 | 2020/09/17 |
| 概要 | Three-dimensional (3D) cardiac tissue reconstruction using tissue engineering technology is a rapidly growing area of regenerative medicine and drug screening model development. Our laboratory has developed a novel drug screening model that measures the contractile force of cardiac cell sheets prepared using temperature-responsive culture dishes. There is an urgent need to measure the contractile force of 3D myocardial tissue close to the living body to accurately predict the drug sensitivity of human heart tissue. However, as difficulties arise during the stacking of cardiomyocyte sheets, the effect of stacking on the contractile force is unknown. We therefore established a method of stacking cell sheets using centrifugal force, making it possible to measure 3D myocardial tissue. Human iPS cell-derived cardiomyocytes were seeded in a temperature-responsive culture dish and processed into a sheet. The cardiac cell sheets were multilayered to construct 3D cardiac tissue. The contractile force and cross-sectional area of the multilayered 3D cardiac tissue were measured, and these measurements were used to determine the relationship between the cross-sectional area of the cardiac tissue and its contractile force. The contractile force of the 1-, 3-, and 5-layer tissues increased linearly in proportion to the cross-sectional area. A result of 6.4 mN/mm2, which is one-seventh of the contractile force found in adult tissue, was obtained. However, with 7-layer tissues, there was a sudden drop in the contractile force, possibly due to limited oxygen and nutrient supply. In conclusion, we established a method wherein the thickness of the cell sheets was controlled via layering, thus enabling accurate evaluation of the cardiac contractile function. This method may enable comparisons to living heart tissue and can provide information applicable to regenerative medicine and drug screening models. |
| DOI | 10.1089/ten.TEC.2020.0164 |
| PMID | 32799760 |