コガ　ヨウコ   KOGA Youko   古賀　陽子    所属   医学部 医学科（東京女子医科大学病院）    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Properties of growth and molecular profiles of rat progenitor cells from ciliary epithelium.
掲載誌名	正式名：Experimental eye research 略　 称：Exp Eye Res ISSNコード：00144835/00144835
掲載区分	国外
巻・号・頁	82(3),pp.471-8
著者・共著者	Yanagi Yasuo, Inoue Yuji, Kawase Yoko, Uchida Saiko, Tamaki Yasuhiro, Araie Makoto, Okochi Hitoshi
発行年月	2006/03
概要	Recent studies have demonstrated that multipotent retinal stem or progenitor cells can be isolated from the ciliary epithelium (CE) of the eye using a neurosphere culture. In this study, we investigated the properties of growth and differentiation, and molecular profiles of rat adult ciliary epithelium (CE)-derived retinal progenitors and forebrain (FB) derived neurospheres. Under clonogenic culture conditions, we found that the CE-derived neurospheres contained fewer undifferentiated cells compared with the FB-derived neurospheres, and that CE-derived neurospheres initially expressed the set of Notch pathway molecules genes including Notch 1 and Delta 1, HES-1 and HES-5, but partially lose their expression after passaging. Furthermore, we found that the CE-derived neurospheres did not express several markers for in vivo embryonic retinal progenitors. Additionally, when the eye was divided into four subregions along its dorsoventral and nasotemporal axes and progenitor cells were obtained from the subregions, the progenitor cells did not express the subregion specific transcription factors, suggesting that subregional specificity is not maintained in vitro. Together, our results demonstrate that CE-derived progenitor cells may have intrinsic limitations in the production of cell types.
DOI	10.1016/j.exer.2005.08.005
PMID	16198338