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コガ ヨウコ
KOGA Youko
古賀 陽子 所属 医学部 医学科（東京女子医科大学病院） 職種 教授・基幹分野長 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth. |
| 掲載誌名 | 正式名：Experimental cell research 略 称：Exp Cell Res ISSNコード：00144827/00144827 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 295(1),pp.194-203 |
| 著者・共著者 | Kawase Yoko, Yanagi Yasuo, Takato Tsuyoshi, Fujimoto Manabu, Okochi Hitoshi |
| 発行年月 | 2004/04 |
| 概要 | Recently, adult stem cells have been isolated from the skin and designated as skin-derived precursors (SKPs). These SKPs, cultured in vitro, can give rise to neurons, glia, smooth muscle cells, and adipocytes. In the current study, we confirmed the clonal expansion of SKPs using a sphere-forming culture system in a medium containing methylcellulose. Among the growth factors, only transforming growth factor-beta (TGF-beta) was revealed to uniquely facilitate the sphere formation and proliferation of the SKPs in combination with EGF and bFGF. In addition, TGF-beta did not alter phenotypical characteristics of the SKPs under sphere-forming conditions. The effect of TGF-beta on sphere formation was not observed in neural stem cells, which expressed a different set of cell surface markers from SKPs, suggesting that SKPs have distinct features. Although the number of SKPs decreased with age, TGF-beta increased the sphere colony formation and proliferation in all ages. These results suggest that SKPs maintained in the presence of TGF-beta during culture are of potential use in cell-replacement therapies employing adult tissue sources. |
| DOI | 10.1016/j.yexcr.2003.12.027 |
| PMID | 15051502 |