|
ウツギサワ タイジユ
UTSUGISAWA Taijiyu
槍澤 大樹 所属 医学部 医学科（東京女子医科大学病院） 職種 准教授 |
|
| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | A road map toward defining the role of Smad signaling in hematopoietic stem cells. |
| 掲載誌名 | 正式名：Stem cells (Dayton, Ohio) 略 称：Stem Cells ISSNコード：10665099/10665099 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 24(4),pp.1128-36 |
| 著者・共著者 | Utsugisawa Taiju, Moody Jennifer L, Aspling Marie, Nilsson Eva, Carlsson Leif, Karlsson Stefan |
| 担当区分 | 筆頭著者 |
| 発行年月 | 2006/04 |
| 概要 | The transforming growth factor-beta (TGF-beta) superfamily encompasses the ligands and receptors for TGF-beta, bone morphogenic proteins (BMPs), and Activins. Cellular response to ligand is context-dependent and may be controlled by specificity and/or redundancy of expression of these superfamily members. Several pathways within this family have been implicated in the proliferation, differentiation, and renewal of hematopoietic stem cells (HSCs); however, their roles and redundancies at the molecular level are poorly understood in the rare HSC. Here we have characterized the expression of TGF-beta superfamily ligands, receptors, and Smads in murine HSCs and in the Lhx2-hematopoietic progenitor cell (Lhx2-HPC) line. We demonstrate a remarkable likeness between these two cell types with regard to expression of the majority of receptors and Smads necessary for the transduction of signals from TGF-beta, BMP, and Activin. We have also evaluated the response of these two cell types to various ligands in proliferation assays. In this regard, primary cells and the Lhx2-HPC line behave similarly, revealing a suppressive effect of Activin-A that is similar to that of TGF-beta in bulk cultures and no effect of BMP-4 on proliferation. Signaling studies that verify the phosphorylation of Smad2 (Activin and TGF-beta) and Smad1/5 (BMP) confirm cytosolic responses to these ligands. In addition to providing a thorough characterization of TGF-beta superfamily expression in HSCs, our results define the Lhx2-HPC line as an appropriate model for molecular characterization of Smad signaling. |
| DOI | 10.1634/stemcells.2005-0263 |
| PMID | 16357343 |