ウツギサワ　タイジユ   UTSUGISAWA Taijiyu   槍澤　大樹    所属   医学部 医学科（東京女子医科大学病院）    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas.
掲載誌名	正式名：Laboratory investigation; a journal of technical methods and pathology 略　 称：Lab Invest ISSNコード：00236837/00236837
掲載区分	国外
巻・号・頁	84(7),pp.932-936
著者・共著者	Uchiyama Michihiro, Maesawa Chihaya, Yashima-Abo Akiko, Tarusawa Mitsu, Satoh Mamoru, Satoh Takashi, Ishida Yoji, Ito Shigeki, Murai Kazunori, Enomoto Sanae, Utsugisawa Taiju, Masuda Tomoyuki
発行年月	2004/07
概要	We used 3'-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD.
DOI	10.1038/labinvest.3700092
PMID	15034598