ウツギサワ　タイジユ   UTSUGISAWA Taijiyu   槍澤　大樹    所属   医学部 医学科（東京女子医科大学病院）    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Quantitative assessment of contaminating tumor cells in autologous peripheral blood stem cells of B-cell non-Hodgkin lymphomas using immunoglobulin heavy chain gene allele-specific oligonucleotide real-time quantitative-polymerase chain reaction.
掲載誌名	正式名：Leukemia research 略　 称：Leuk Res ISSNコード：01452126/01452126
掲載区分	国外
巻・号・頁	27(10),pp.925-934
著者・共著者	Yashima Akiko, Maesawa Chihaya, Uchiyama Michihiro, Tarusawa Mitsu, Satoh Takashi, Satoh Mamiko, Enomoto Sanae, Sugawara Ken, Numaoka Hideharu, Murai Kazunori, Utsugisawa Taiju, Ishida Yoji, Masuda Tomoyuki
発行年月	2003/10
概要	A real-time quantitative-polymerase chain reaction (RQ-PCR) targeting the immunoglobulin heavy chain (IgH) gene has been used for the quantification of minimal residual disease (MRD) in B-cell hematological malignancies. In non-Hodgkin lymphoma (NHL), experimental costs are increased, as a large number of primer-probe sets are required because of diversity, due to somatic and ongoing mutations of the IgH gene. We developed an allele-specific oligonucleotide (ASO) combined with a germline consensus probe-based RQ-PCR assay and examined MRD in peripheral blood stem cells (PBSC). The IgH consensus probes were adapted in seven (50%) of 14 amplifiable cases. Patients with heavily contaminating tumor cells in PBSC relapsed after PBSC transplantation. Our strategy will contribute to the development of a cost-efficient, precisely quantitative and systemic detection assay for MRD in NHL.
DOI	10.1016/s0145-2126(03)00049-3
PMID	12860013