ナカヤマ　マサミチ   Nakayama Masamichi   中山　正道    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Water Stable Nanocoatings of Poly(N-isopropylacrylamide)-based Block Copolymers on Culture Insert Membrane for Temperature-Controlled Cell Adhesion
掲載誌名	正式名：Journal of materials chemistry. B. 略　 称：J Mater Chem B ISSNコード：2050750X/20507518
掲載区分	国外
出版社	Royal Society of Chemistry
巻・号・頁	8(34),pp.7812-7821
著者・共著者	NAKAYAMA Masamichi†*, TOYOSHIMA Yuki, CHINEN Hiroshi, KIKUCHI Akihiko, YAMATO Masayuki, OKANO Teruo
担当区分	筆頭著者,責任著者
発行年月	2020/07/17
概要	This study demonstrated the spin-coating of functional diblock copolymers to develop smart culture inserts for thermoresponsive cell adhesion/detachment control. One part of the block components, the poly(n-butyl methacrylate) block, strongly supported the water stable surface-immobilization of the thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) block, regardless of temperature. The chain length of the PNIPAAm blocks was varied to regulate thermal surface functions. Immobilized PNIPAAm concentrations became larger with increasing chain length (1.0–1.6 μg cm−2) and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers.
DOI	10.1039/D0TB01113D