カワシマ　アキツグ   Kawashima Akitsugu   川島　明次    所属   医学部 医学科（東京女子医科大学病院）    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms.
掲載誌名	正式名：Scientific reports 略　 称：Sci Rep ISSNコード：20452322/20452322
掲載区分	国外
巻・号・頁	9(1),pp.10387
著者・共著者	Aoki Tomohiro, Koseki Hirokazu, Miyata Haruka, Itoh Masayoshi, Kawaji Hideya, Takizawa Katsumi, Kawashima Akitsugu, Ujiie Hiroshi, Higa Takashi, Minamimura Kenzo, Kimura Toshikazu, Kasuya Hidetoshi, Nozaki Kazuhiko, Morita Akio, Sano Hirotoshi, Narumiya Shuh
発行年月	2019/07
概要	Intracranial aneurysm (IA) is a socially important disease as a major cause of subarachnoid hemorrhage. Recent experimental studies mainly using animal models have revealed a crucial role of macrophage-mediated chronic inflammatory responses in its pathogenesis. However, as findings from comprehensive analysis of unruptured human IAs are limited, factors regulating progression and rupture of IAs in humans remain unclear. Using surgically dissected human unruptured IA lesions and control arterial walls, gene expression profiles were obtained by RNA sequence analysis. RNA sequencing analysis was done with read count about 60~100 million which yielded 6~10 billion bases per sample. 79 over-expressed and 329 under-expressed genes in IA lesions were identified. Through Gene Ontology analysis, 'chemokine activity', 'defense response' and 'extracellular region' were picked up as over-represented terms which included CCL3 and CCL4 in common. Among these genes, quantitative RT-PCR analysis using another set of samples reproduced the above result. Finally, increase of CCL3 protein compared with that in control arterial walls was clarified in IA lesions. Findings of the present study again highlight importance of macrophage recruitment via CCL3 in the pathogenesis of IA progression.
DOI	10.1038/s41598-019-46886-2
PMID	31316152