ナギ　サチヨ   Nagi Sachiyo   凪　幸世    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay.
掲載誌名	正式名：PLoS neglected tropical diseases 略　 称：PLoS Negl Trop Dis ISSNコード：19352735/19352727
掲載区分	国外
巻・号・頁	20(9(8)),pp.e0004021
著者・共著者	Tanigawa Chihiro, Fujii Yoshito, Miura Masashi, Nzou Samson Muuo, Mwangi Anne Wanjiru, Nagi Sachiyo, Hamano Shinjiro, Njenga Sammy M, Mbanefo Evaristus Chibunna, Hirayama Kenji, Mwau Matilu, Kaneko Satoshi
発行年月	2015/08
概要	BACKGROUND:Both Schistosoma mansoni and Schistosoma haematobium cause schistosomiasis in sub-Saharan Africa. We assessed the diagnostic value of selected Schistosoma antigens for the development of a multiplex serological immunoassay for sero-epidemiological surveillance.METHODOLOGY/PRINCIPAL FINDINGS:Diagnostic ability of recombinant antigens from S. mansoni and S. haematobium was assessed by Luminex multiplex immunoassay using plasma from school children in two areas of Kenya, endemic for different species of schistosomiasis. S. mansoni serine protease inhibitor (SERPIN) and Sm-RP26 showed significantly higher reactivity to patient plasma as compared to the control group. Sm-Filamin, Sm-GAPDH, Sm-GST, Sm-LAP1, Sm-LAP2, Sm-Sm31, Sm-Sm32 and Sm-Tropomyosin did not show difference in reactivity between S. mansoni infected and uninfected pupils. Sm-RP26 was cross-reactive to plasma from S. haematobium patients, whereas Sm-SERPIN was species-specific. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. ROC analysis for Sm-RP26, Sm-SERPIN and Sh-SERPIN showed AUC values of 0.833, 0.888 and 0.947, respectively. Using Spearman's rank correlation coefficient analysis, we also found significant positive correlation between the number of excreted eggs and median fluorescence intensity (MFI) from the multiplex immunoassays for Sm-SERPIN (ρ = 0.430, p-value = 0.003) and Sh-SERPIN (ρ = 0.433, p-value = 0.006).CONCLUSIONS/SIGNIFICANCE:Sm-SERPIN is a promising species-specific diagnostic antigen. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. SERPINs showed correlation with the number of excreted eggs. These indicate prospects for inclusion of SERPINs in the multiplex serological immunoassay system.
DOI	10.1371/journal.pntd.0004021
PMID	26291988