ニワ　ユキコ   NIWA Yukiko   丹羽　由紀子    所属   医学部 医学科（附属八千代医療センター）    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer.
掲載誌名	正式名：International journal of cancer 略　 称：Int J Cancer ISSNコード：10970215/00207136
掲載区分	国外
巻・号・頁	139(10),pp.2290-8
著者・共著者	Kanda Mitsuro, Tanaka Chie, Kobayashi Daisuke, Tanaka Haruyoshi, Shimizu Dai, Shibata Masahiro, Takami Hideki, Hayashi Masamichi, Iwata Naoki, Niwa Yukiko, Yamada Suguru, Fujii Tsutomu, Nakayama Goro, Fujiwara Michitaka, Kodera Yasuhiro
発行年月	2016/11
概要	Prediction of tumor recurrence after curative resection is critical for determining the prognosis of patients with gastric cancer (GC). The initiation and progression of GC are associated with inappropriate immune responses caused by chronic inflammation of the gastric mucosa. To identify immunoregulatory molecules involved in GC progression, GC cell lines and 200 pairs of tumor and normal tissues from patients with GC were analyzed for gene expression, amplification and methylation as well as function of a differentially expressed gene. The transcriptome analysis revealed that marginal zone B and B1 cell specific protein (MZB1) was expressed at significantly decreased levels in primary GC tissues when compared with the corresponding normal gastric mucosa. PCR array analysis exploring genes expressed cooperatively with MZB1 revealed that differential expression of MZB1 mRNA in GC cell lines correlated positively with the levels of the mRNAs encoding estrogen receptor 1 and desumoylating isopeptidase 1. Hypermethylation of the MZB1 promoter was frequent in cell lines with decreased levels of MZB1 mRNA. siRNA-mediated knockdown of MZB1 significantly increased proliferation, invasion and migration of GC cell lines. Low MZB1 expression was an independent prognostic factor for recurrence after curative gastrectomy and was associated significantly with increased hematogenous recurrence. MZB1 acts as a suppressor of GC. Low MZB1 expression in the primary GC tissue is predictive of recurrence after curative resection.
DOI	10.1002/ijc.30286
PMID	27459504