イワサキ　マサユキ   Iwasaki Masayuki   岩崎　正幸    所属   研究施設 研究施設    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	A mandatory role for STAT4 in IL-12 induction of mouse T cell CCR5.
掲載誌名	正式名：Journal of immunology (Baltimore, Md. : 1950) 略　 称：J Immunol ISSNコード：00221767/00221767
掲載区分	国外
巻・号・頁	167(12),pp.6877-83
著者・共著者	Iwasaki M, Mukai T, Nakajima C, Yang Y F, Gao P, Yamaguchi N, Tomura M, Fujiwara H, Hamaoka T
担当区分	筆頭著者
発行年月	2001/12
概要	IL-12 was recently shown to induce CCR5 on TCR-triggered mouse T cells. Considering that STAT4 is the most critical of IL-12 signaling molecules, this study investigated the role for STAT4 in the induction of CCR5 expression. IL-12R was induced by stimulation with anti-CD3 plus anti-CD28 mAb similarly on T cells from wild-type (WT) and STAT4-deficient (STAT4(-/-)) mice, but the levels of IL-12R induced on IFN-gamma-deficient (IFN-gamma(-/-)) T cells were lower compared with WT T cells. Exposure of TCR-triggered WT T cells to IL-12 induced CCR5 expression. In contrast, TCR-triggered STAT4(-/-) T cells failed to express CCR5 in response to IL-12. IL-12 stimulation induced detectable albeit reduced levels of CCR5 expression on IFN-gamma(-/-) T cells. Addition of rIFN-gamma to cultures of IFN-gamma(-/-) T cells, particularly to cultures during TCR triggering resulted in restoration of CCR5 expression. However, CCR5 expression was not induced in STAT4(-/-) T cells by supplementation of rIFN-gamma. These results indicate that for the induction of CCR5 on T cells, 1) STAT4 plays an indispensable role; 2) such a role is not substituted by simply supplementing rIFN-gamma; and 3) IFN-gamma amplifies CCR5 induction depending on the presence of STAT4.
DOI	10.4049/jimmunol.167.12.6877
PMID	11739505