トキタ　ダイスケ   Tokita Daisuke   時田　大輔    所属   医学部 医学科（東京女子医科大学病院）    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Poor allostimulatory function of liver plasmacytoid DC is associated with pro-apoptotic activity, dependent on regulatory T cells.
掲載誌名	正式名：Journal of hepatology 略　 称：J Hepatol ISSNコード：01688278/01688278
掲載区分	国外
巻・号・頁	49(6),pp.1008-18
著者・共著者	Tokita Daisuke, Sumpter Tina L, Raimondi Giorgio, Zahorchak Alan F, Wang Zhiliang, Nakao Atsunori, Mazariegos George V, Abe Masanori, Thomson Angus W
担当区分	筆頭著者
発行年月	2008/12
概要	BACKGROUND/AIMS:The liver is comparatively rich in plasmacytoid (p) dendritic cells (DC), - innate immune effector cells that are also thought to play key roles in the induction and regulation of adaptive immunity.METHODS:Liver and spleen pDC were purified from fms-like tyrosine kinase ligand-treated control or lipopolysaccharide-injected C57BL/10 mice. Flow cytometric and molecular biologic assays were used to characterize their function and interaction with naturally occurring regulatory T cells (Treg).RESULTS:While IL-10 production was greater for freshly isolated liver compared with splenic pDC, the former produced less bioactive IL-12p70. Moreover, liver pDC expressed a low Delta4/Jagged1 Notch ligand ratio, skewed towards T helper 2 cell differentiation/cytokine production, and promoted allogeneic CD4(+)T cell apoptosis. T cell proliferation in response to liver pDC was, however, enhanced by blocking IL-10 function at the initiation of cultures. In the absence of naturally occurring CD4(+)CD25(+) regulatory T cells, similar levels of T cell proliferation were induced by liver and spleen pDC and the pro-apoptotic activity of liver pDC was reversed.CONCLUSIONS:The inferior T cell allostimulatory activity of in vivo-stimulated liver pDC may depend on the presence and function of Treg, a property that may contribute to inherent liver tolerogenicity.
DOI	10.1016/j.jhep.2008.07.028
PMID	18926588