オノ　マサフミ   Ono Masafumi   小野　正文    所属   医学部 医学科（附属足立医療センター）    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Co-administration of insulin with a gonadotropin partly improves ovulatory responses of estrogen-deficient mice.
掲載誌名	正式名：Molecular and cellular endocrinology 略　 称：Mol Cell Endocrinol ISSNコード：18728057/03037207
掲載区分	国外
巻・号・頁	411,pp.177-86
著者・共著者	Toda Katsumi, Hayashi Yoshihiro, Ono Masafumi, Saibara Toshiji
発行年月	2015/08
概要	Administration of 17-βestradiol (E2) with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) can induce ovulation in estrogen-deficient (ArKO) mice; nevertheless, ovulatory efficiency and rate are low. In this study, effects of insulin on the ovulatory responses were investigated. In ArKO ovary, hCG signal was found to be transmitted in an uncoordinated manner when phosphorylation levels of signaling molecules are examined. Co-administration of insulin with hCG improved the transmission of hCG signal as well as the ovulatory efficiency in ArKO mice. It also improved the ovulatory rate but far below the wild-type rate. Gene expression analysis demonstrated that Cyp11a1 and Cyp17a1 mRNAs were significantly induced 4 h after PMSG administration in the wild-type ovary, but not in ArKO ovary. Collectively, these results suggest that insulin improves ovulatory responses of ArKO mice, but it fails to ameliorate follicular dysfunctions caused possibly by an inappropriate intraovarian milieu during follicular maturation.
DOI	10.1016/j.mce.2015.04.027
PMID	25957088