イマシロ　チカヒロ   Imashiro Chikahiro   今城　哉裕    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves.
掲載誌名	正式名：Communications biology 略　 称：Commun Biol ISSNコード：23993642/23993642
掲載区分	国外
巻・号・頁	2,pp.393
著者・共著者	Kurashina Yuta†*, Imashiro Chikahiro, Hirano Makoto, Kuribara Taiki, Totani Kiichiro, Ohnuma Kiyoshi, Friend James, Takemura Kenjiro*
発行年月	2019/10/29
概要	Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free cell detachment method that employs the acoustic pressure, sloshing in serum-free medium from intermittent traveling wave. This method detaches 96.2% of the cells, and increases its transfer yield to 130% of conventional methods for 48 h, compared to the number of cells detached by trypsinization. We show the elimination of trypsinization reduces cell damage, improving the survival of the detached cells. Acoustic pressure applied to the cells and media sloshing from the intermittent traveling wave were identified as the most important factors leading to cell detachment. This proposed method will improve biopharmaceutical production by expediting the amplification of tissue-cultured cells through a more efficient transfer process.
DOI	10.1038/s42003-019-0638-5
PMID	31701022