イマシロ　チカヒロ   Imashiro Chikahiro   今城　哉裕    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated From Resonance Vibration of a Disk-Shaped Ultrasonic Transducer.
掲載誌名	正式名：IEEE transactions on bio-medical engineering 略　 称：IEEE Trans Biomed Eng ISSNコード：15582531/00189294
掲載区分	国外
巻・号・頁	66(1),pp.111-118
著者・共著者	Imashiro Chikahiro†, Kurashina Yuta, Kuribara Taiki, Hirano Makoto, Totani Kiichiro, Takemura Kenjiro*
発行年月	2019/01
概要	Cell patterning methods have been previously reported for cell culture. However, these methods use inclusions or devices that are not used in general cell culture and that might affect cell functionality. Here, we report a cell patterning method that can be conducted on a general cell culture dish without any inclusions by employing a resonance vibration of a disk-shaped ultrasonic transducer located under the dish. A resonance vibration with a single nodal circle patterned C2C12 myoblasts into a circular shape on the dish with 10-min exposure of the vibration with maximum peak-peak amplitude of 10 μm[Formula: see text]. Furthermore, the relationship between the amplitude distribution of the transducer and the cell density in the patterned sample could be expressed as a linear function, and there was a clear threshold of amplitude for cell adhesion. To evaluate the cell function of the patterned cells, we conducted proliferation and protein assays at 120-h culture after patterning. Our results showed that the cell proliferation rate did not decrease and the expression of cellular proteins was unchanged. Thus, we conclude, this method can successfully pattern cells in the clinically ubiquitous culture dish, while maintaining cell functionality.
DOI	10.1109/TBME.2018.2835834
PMID	29993416