イタバシ　ミチオ   Itabashi Michio   板橋　道朗    所属   医学部 医学科    職種   特任教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study.
掲載誌名	正式名：Annals of surgical oncology 略　 称：Ann Surg Oncol ISSNコード：15344681/10689265
巻・号・頁	27(4),pp.1077-1083
著者・共著者	Itabashi Michio, Yamamoto Hirofumi, Tomita Naohiro, Inomata Masafumi, Murata Kohei, Hayashi Shigeoki, Miyake Yasuhiro, Igarashi Seiji, Kato Takeshi, Noura Shingo, Furuhata Tomohisa, Ozawa Heita, Takemasa Ichiro, Yasui Masayoshi, Takeyama Hiroshi, Okamura Shu, Ohno Yuko, Matsuura Nariaki
担当区分	筆頭著者
発行年月	2020/04
概要	BACKGROUND:For colorectal cancer (CRC) patients, the standard histological lymph node (LN) evaluation has low sensitivity. Our previously developed one-step nucleic acid amplification (OSNA™) assay measures cytokeratin 19 gene expression in whole LNs. We recently showed that 17.6% of pN0 stage II CRC patients were OSNA positive, suggesting a correlation between OSNA results and disease recurrence. This multicenter, prospective study investigateed the prognostic value of the OSNA assay for pStage II CRC patients.METHODS:We examined 204 CRC patients who were preoperatively diagnosed as cN0 and cN1 and surgically treated at 11 medical institutions across Japan. Nine patients were excluded, and 195 patients (Stage I: n = 50, Stage II: n = 70, Stage III: n = 75) were examined. All LNs, harvested from patients, were examined histopathologically using one-slice hematoxylin-eosin staining. Furthermore, half of the LNs was examined by the OSNA assay. Patients were classified according to the UICC staging criteria and OSNA results, and the 3-year, disease-free survival (DFS) of each cohort was analyzed.RESULTS:Average 21.2 LNs/patient were subject to pathological examination. Approximately half of all harvested LNs (average, 9.4 LNs/patient) were suitable for the OSNA assay. Significantly lower 3-year DFS rates were observed in pStage (pathological Stage) II OSNA-positive patients than in OSNA-negative patients (p = 0.005). Among all assessed clinical and pathological parameters, only the OSNA result significantly affected 3-year DFS rates in pStage II CRC patients (p = 0.027).CONCLUSIONS:This study shows that OSNA positivity is a risk factor for recurrence of the patients with pStage II CRC.
DOI	10.1245/s10434-019-07971-y
PMID	31722072