オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip.
掲載誌名	正式名：Biomedical microdevices 略　 称：Biomed Microdevices ISSNコード：15728781/13872176
掲載区分	国外
出版社	Springer
巻・号・頁	22(1),pp.9
著者・共著者	TAKEHARA Hiroaki†*, SAKAGUCHI Katsuhisa, SEKINE Hidekazu, OKANO Teruo, SHIMIZU Tatsuya*
発行年月	2019/12/20
概要	In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections between endothelial capillaries of heterogeneous sections (5-100 μm in diameter) and artificial plastic tubes or reservoirs (1-10 mm in diameter). Moreover, the micro-VB device can be installed in a standard 100 mm-diameter Petri dish. Endothelial networks in 3D engineered tissues were obtained by cellular self-assembly on the device, after co-culturing of human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) in fibrin gel. Endothelial capillary connection between vascularized tissues and microfluidic channels, mimicking arteries and veins, was confirmed by perfusion of fluorescent microspheres. The micro-VB devices were compatible with the use of commercially available culture dishes and did not require the involvement of additional equipment. Thus, these micro-VB devices are expected to substantially improve the routine application of 3D tissue engineering to regenerative medicine.
DOI	10.1007/s10544-019-0461-2
PMID	31863202