タキタ　モリチカ   Takita Morichika   瀧田　守親    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Lung Tumor Cell Recruitment Assay.
掲載誌名	正式名：Journal of visualized experiments : JoVE 略　 称：J Vis Exp ISSNコード：1940-087X/1940-087X
掲載区分	国外
巻・号・頁	(144),pp.e53172
著者・共著者	Tomita Takeshi, Ieguchi Katsuaki, Deguchi Atsuko, Takita Morichika, Tsukahara Fujiko, Hiratsuka Sachie, Maru Yoshiro
発行年月	2019/02
概要	To investigate the molecular mechanisms governing tumor metastasis, various assays using the mouse as a model animal have been proposed. Here, we demonstrate a simple assay to evaluate tumor cell extravasation or micrometastasis. In this assay, tumor cells were injected through the tail vein, and after a short period, the lungs were dissected and digested to count the accumulated labeled tumor cells. This assay skips the initial step of primary tumor invasion into the blood vessel and facilitates the study of events in the distant organ where tumor metastasis occurs. The number of cells injected into the blood vessel can be optimized to observe a limited number of metastases. It has been reported that stromal cells in the distant organ contribute to metastasis. Thus, this assay could be a useful tool to explore potential therapeutic drugs or devices for prevention of tumor metastasis.
DOI	10.3791/53172
PMID	30882774
researchmap用URL	https://www.jove.com/t/53172/lung-tumor-cell-recruitment-assay