オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Developing palatal bone using human mesenchymal stem cell and stem cells from exfoliated deciduous teeth cell sheets
掲載誌名	正式名：Journal of tissue engineering and regenerative medicine 略　 称：J Tissue Eng Regen Med ISSNコード：(1932-7005)1932-6254(Linking)
掲載区分	国外
出版社	Wiley
巻・号・頁	13(2),pp.319-327
著者・共著者	LEE Jong-Min†, KIM Hyun-Yi, PARK Jin-Sung, LEE Dong-Joon, ZHANG Sushan, GREEN David William, OKANO Teruo, HONG Jeong-Ho, JUNG Han-Sung*
発行年月	2019/02
概要	Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only esthetic but also functional problems with speech, eating and breathing. Bone grafts using autologous cancellous bone have been a standard treatment to repair the hard tissue defect in cleft palates. However, such grafts do not fully integrate into host bone and undergo resorption. To overcome engraftment problems, it is common to engineer new tissues with a combination of multipotent cells and biomaterial frameworks. Here, we manufactured cell sheets for bone repair of cleft palates derived from two osteogenic cell sources, human mesenchymal stem cells (hMSC) and stem cells from human exfoliated deciduous teeth (SHED). Cell sheets made from hMSCs and SHEDs gave rise to in vitro calcification, which indicated the osteogenic potential of these cells. The cell sheets of hMSCs and SHEDs expressed the bone-specific osteogenic markers, Osterix (OSX), Osteocalcin (OCN) and Osteopontin (OPN), following insertion into ex vivo-cultured embryonic palatal shelves and in ovo culture. In conclusion, we showed that osteogenic stem cell sheets have mineralization potential and might represent a new alternative to autologous bone transplantation in the reconstruction of cleft palates.
DOI	10.1002/term.2811
PMID	30644640