ハラグチ　ユウジ   Haraguchi Yuuji   原口　裕次    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   特任准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by centrifugation.
掲載誌名	正式名：Biotechnology progress 略　 称：Biotechnol Prog ISSNコード：(1520-6033)1520-6033(Linking)
掲載区分	国外
出版社	Wiley
巻・号・頁	34(6),pp.1447-1453
著者・共著者	HARAGUCHI Yuji†, MATSUURA Katsuhisa, KAGAWA Yuki, HASEGAWA Akiyuki, KUBO Hirotsugu, SHIMIZU Tatsuya*
担当区分	筆頭著者
発行年月	2018/12
概要	This study reports a rapid fabrication system of a morphologically and functionally communicative 3-D cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37°C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 µm-thickness were created at 1.5 h [centrifugation (80 x g, 37°C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell-derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37°C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. This article is protected by copyright. All rights reserved.
DOI	10.1002/btpr.2691
PMID	30009512