フルヤ　タケフミ   Furuya Takefumi   古谷　武文    所属   医学部 医学科（東京女子医科大学病院）    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	IFN-gamma-producing human T cells directly induce osteoclastogenesis from human monocytes via the expression of RANKL.
掲載誌名	正式名：European journal of immunology 略　 称：Eur J Immunol ISSNコード：(0014-2980)0014-2980(Linking)
掲載区分	国外
巻・号・頁	35(11),pp.3353-3363
著者・共著者	Kotake Shigeru, Nanke Yuki, Mogi Makio, Kawamoto Manabu, Furuya Takefumi, Yago Toru, Kobashigawa Tsuyoshi, Togari Akifumi, Kamatani Naoyuki
発行年月	2005/11
概要	The current study explored our hypothesis that IFN-gamma-producing human T cells inhibit human osteoclast formation. Activated T cells derived from human PBMC were divided into IFN-gamma-producing T cells (IFN-gamma(+) T cells) and IFN-gamma-non-producing T cells (IFN-gamma(-) T cells). IFN-gamma(+) T cells were cultured with human monocytes in the presence of macrophage-CSF alone. The concentration of soluble receptor activator of NF-kappaB ligand (RANKL) and IFN-gamma, and the amount of membrane type RANKL expressed on T cells, were measured by ELISA. In the patients with early rheumatoid arthritis (RA) treated with non-steroidal anti-inflammatory drugs alone, CD4+ T cells expressing both IFN-gamma and RANKL were detected by flow cytometry. Surprisingly, IFN-gamma(+) T cells, but not IFN-gamma(-) T cells, induced osteoclastogenesis from monocytes, which was completely inhibited by adding osteoprotegerin and increased by adding anti-IFN-gamma antibodies. The levels of both soluble and membrane type RANKL were elevated in IFN-gamma(+) T cells. The ratio of CD4+ T cells expressing both IFN-gamma and RANKL in total CD4+ T cells from PBMC was elevated in RA patients. Contrary to our hypothesis, IFN-gamma(+) human T cells induced osteoclastogenesis through the expression of RANKL, suggesting that Th1 cells play a direct role in bone resorption in Th1 dominant diseases such as RA.
DOI	10.1002/eji.200526141
PMID	16220542