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ヤマウチ カヅヨ
YAMAUCHI Kadzuyo
山内 かづ代 所属 医学部 医学科 職種 評議員 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Inhibiting nerve growth factor or its receptors downregulates calcitonin gene-related peptide expression in rat lumbar dorsal root ganglia innervating injured intervertebral discs. |
| 掲載誌名 | 正式名：Journal of orthopaedic research : official publication of the Orthopaedic Research Society 略 称：J Orthop Res ISSNコード：(1554-527X)0736-0266(Linking) |
| 掲載区分 | 国外 |
| 巻・号・頁 | 28(12),pp.1614-20 |
| 著者・共著者 | Orita Sumihisa, Ohtori Seiji, Nagata Maiko, Horii Manato, Yamashita Masaomi, Yamauchi Kazuyo, Inoue Gen, Suzuki Munetaka, Eguchi Yawara, Kamoda Hiroto, Arai Gen, Ishikawa Tetsuhiro, Miyagi Masayuki, Ochiai Nobuyasu, Kishida Shunji, Takaso Masashi, Aoki Yasuchika, Takahashi Kazuhisa |
| 発行年月 | 2010/12 |
| 概要 | Nerve growth factor (NGF) and its dual structurally unrelated receptors, tropomyosin-related kinase A (TrkA) or p75 neurotrophin receptor (p75(NTR)), cause the pathogenesis of discogenic pain. To investigate the sensory innervation of injured rat lumbar intervertebral disc (IVD), we examined the expression of neuropeptides such as calcitonin gene-related peptide (CGRP) at dorsal root ganglia (DRG) by inhibiting NGF or its dual receptors. Sprague-Dawley rats with multiply punctured L5-L6 IVD were used. Six experimental groups were prepared: naïve, sham control, and four agent-treated groups with punctured IVD (vehicle, anti-NGF antibody, anti-TrkA antibody, and anti-p75(NTR) antibody). Retrograde neurotracer Fluoro-Gold (FG) was applied together except for the naïve group. Their lumbar DRG were harvested and immunolabeled for CGRP. FG-labeled DRG neurons were most prevalent at L1 and L2 DRG, and the proportion of FG-labeled CGRP-immunoreactive DRG neurons in the vehicle group was significantly elevated (p < 0.05) compared with the sham group, while those of antibody-treated groups, especially in the anti-p75(NTR) group, significantly decreased compared with the vehicle group (p < 0.05). Direct intradiscal application of antibody to NGF or its receptors suppressed CGRP expression, and p75(NTR) antagonism induced the most profound suppression. |
| DOI | 10.1002/jor.21170 |
| PMID | 20973063 |