ヤマウチ　カヅヨ   YAMAUCHI Kadzuyo   山内　かづ代    所属   医学部 医学科    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Evaluation of behavior and neuropeptide markers of pain in a simple, sciatic nerve-pinch pain model in rats.
掲載誌名	正式名：European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 略　 称：Eur Spine J ISSNコード：(1432-0932)0940-6719(Linking)
掲載区分	国外
巻・号・頁	19(10),pp.1746-52
著者・共著者	Hirose Kazutoshi, Iwakura Nahoko, Orita Sumihisa, Yamashita Masaomi, Inoue Gen, Yamauchi Kazuyo, Eguchi Yawara, Ochiai Nobuyasu, Kishida Shunji, Nakamura Junichi, Takaso Masashi, Ishikawa Tetsuhiro, Arai Gen, Miyagi Masayuki, Kamoda Hiroto, Aoki Yasuchika, Hiwatari Ryo, Kakizaki Jun, Kunishi Toshikazu, Kono Motoaki, Suzuki Takane, Toyone Tomoaki, Takahashi Kazuhisa, Kuniyoshi Kazuki, Ohtori Seiji
発行年月	2010/10
概要	Pathomechanisms of injured-nerve pain have not been fully elucidated. Radicular pain and chronic constriction injury models have been established; however, producing these models is complicated. A sciatic nerve-pinch injury is easy to produce but the reliability of this model for evaluating pain behavior has not been examined. The current study evaluated pain-related behavior and change in pain markers in the dorsal root ganglion (DRG) of rats in a simple, sciatic nerve-pinch injury model. In the model, the sciatic nerve was pinched for 2 s using forceps (n = 20), but not injured in sham-operated animals (n = 20). Mechanical and thermal hyperalgesia were measured every second day for 2 weeks using von Frey filaments and a Hargreaves device. Calcitonin gene-related peptide (CGRP), activating transcription factor-3 (ATF-3), phosphorylated p38 mitogen activated protein (Map) kinase (p-p38), and nuclear factor-kappa B (NF-κB; p65) expression in L5 DRGs were examined at 4 and 7 days after surgery using immunohistochemistry. The proportion of neurons immunoreactive for these markers was compared between the two groups. Mechanical (during 8 days) and thermal hyperalgesia (during 6 days) were found in the pinch group rats, but not in the sham-operated animals (p < 0.05); however, hyperalgesia was not significant from days 10 to 14. CGRP, ATF-3, p-p38, and NF-κB expression in L5 DRGs was upregulated in the nerve-injured rats compared with the sham-operated rats (p < 0.01). Our results indicate that a simple sciatic nerve pinch produced pain-related behavior. Upregulation of the pain-marker expression in the nerve-injury model suggested it could be used as a model of pain. However, it was not considered as suitable for long-term studies.
DOI	10.1007/s00586-010-1428-4
PMID	20490875