ヤマウチ　カヅヨ   YAMAUCHI Kadzuyo   山内　かづ代    所属   医学部 医学科    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Freeze-Dried Platelet-Rich Plasma Accelerates Bone Union with Adequate Rigidity in Posterolateral Lumbar Fusion Surgery Model in Rats.
掲載誌名	正式名：Scientific reports 略　 称：Sci Rep ISSNコード：(2045-2322)2045-2322(Linking)
掲載区分	国外
巻・号・頁	6,pp.36715
著者・共著者	Shiga Yasuhiro, Orita Sumihisa, Kubota Go, Kamoda Hiroto, Yamashita Masaomi, Matsuura Yusuke, Yamauchi Kazuyo, Eguchi Yawara, Suzuki Miyako, Inage Kazuhide, Sainoh Takeshi, Sato Jun, Fujimoto Kazuki, Abe Koki, Kanamoto Hirohito, Inoue Masahiro, Kinoshita Hideyuki, Aoki Yasuchika, Toyone Tomoaki, Furuya Takeo, Koda Masao, Takahashi Kazuhisa, Ohtori Seiji
発行年月	2016/11
概要	Fresh platelet-rich plasma (PRP) accelerates bone union in rat model. However, fresh PRP has a short half-life. We suggested freeze-dried PRP (FD-PRP) prepared in advance and investigated its efficacy in vivo. Spinal posterolateral fusion was performed on 8-week-old male Sprague-Dawley rats divided into six groups based on the graft materials (n = 10 per group): sham control, artificial bone (A hydroxyapatite-collagen composite) -alone, autologous bone, artificial bone + fresh-PRP, artificial bone + FD-PRP preserved 8 weeks, and artificial bone + human recombinant bone morphogenetic protein 2 (BMP) as a positive control. At 4 and 8 weeks after the surgery, we investigated their bone union-related characteristics including amount of bone formation, histological characteristics of trabecular bone at remodeling site, and biomechanical strength on 3-point bending. Comparable radiological bone union was confirmed at 4 weeks after surgery in 80% of the FD-PRP groups, which was earlier than in other groups (p < 0.05). Histologically, the trabecular bone had thinner and more branches in the FD-PRP. Moreover, the biomechanical strength was comparable to that of autologous bone. FD-PRP accelerated bone union at a rate comparable to that of fresh PRP and BMP by remodeling the bone with thinner, more tangled, and rigid trabecular bone.
DOI	10.1038/srep36715
PMID	27833116