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シミズ タツヤ
Shimizu Tatsuya
清水 達也 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 教授 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Mesenchymal stem cell sheets exert anti-stenotic effects in a rat arterial injury model. |
| 掲載誌名 | 正式名：Tissue engineering. Part A. 略 称：Tissue Eng Part A ISSNコード：19373341/1937335X |
| 掲載区分 | 国外 |
| 巻・号・頁 | 24(19-20),pp.1545-1553 |
| 著者・共著者 | HOMMA Jun†, SEKINE Hidekazu*, MATSUURA Katsuhisa, SHIMIZU Tatsuya |
| 担当区分 | 最終著者 |
| 発行年月 | 2018/10 |
| 概要 | Restenosis after catheter or surgical intervention substantially affects the prognosis of arterial occlusive disease. Mesenchymal stem cells (MSCs) may have anti-stenotic effects on injured arteries. MSC transplantation from the adventitial side of an artery is safer than endovascular transplantation but has not been extensively examined. In this study, a rat model of femoral artery injury was used to compare the anti-stenotic effects of transplanted cell sheets and transplanted cell suspensions. Rat adipose-derived stem cells (ASCs) were used as the source of MSCs. For both cell sheets and suspensions, 6×106 MSCs were transplanted on the day of arterial injury. MSC sheets attenuated neointimal hyperplasia more than MSC suspensions (intima-to-media ratio in haematoxylin/eosin-stained sections: 0.55±0.13 vs. 1.14±0.12; P<0.05). Cell engraftment (assessed by immunohistochemistry or bioluminescence imaging of luciferase-expressing cells), arterial re-endothelialisation (evaluated by immunohistochemical staining for rat endothelial cell antigen-1) and restriction of vascular smooth muscle cell proliferation in the neointima (double-staining of alpha-smooth muscle actin and phospho-histone H3) were greater when MSC sheets were applied than when MSC suspensions were used. In conclusion, MSC sheets exhibited better anti-stenotic and cell engraftment properties than MSC suspensions. MSC sheet transplantation from the adventitial side is a promising therapy for prevention of arterial restenosis. |
| DOI | 10.1089/ten.TEA.2018.0030 |
| PMID | 29724149 |