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ヤマト マサユキ
Yamato Masayuki
大和 雅之 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 教授 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Diverse functions of secreted frizzled-related proteins in the osteoblastogenesis of human multipotent mesenchymal stromal cells. |
| 掲載誌名 | 正式名：Biomaterials 略 称：Biomaterials ISSNコード：(1878-5905)0142-9612(Linking) |
| 掲載区分 | 国外 |
| 巻・号・頁 | 34(13),pp.3270-3278 |
| 著者・共著者 | Yamada Azusa†, Iwata Takanori*, Yamato Masayuki, Okano Teruo, Izumi Yuichi |
| 発行年月 | 2013/04 |
| 概要 | Osteoinductive pretreatment of human mesenchymal stromal cells (hMSCs) has been widely accepted in bone tissue engineering before the use of cell transplantation; however, the mechanisms by which osteoinductive medium (OIM) enhances osteoblastic differentiation are not well understood. Using periodontal ligament-derived hMSCs, we identified key signalling molecules for osteoblastogenesis. Alkaline phosphatase activity induced by OIM, which contains ascorbic acid, β-glycerophosphate, and dexamethasone, was decreased by XAV939, which is an inhibitor of canonical WNT signalling, in a dose-dependent manner. A quantitative RT-PCR array demonstrated the upregulation of secreted frizzled-related protein (SFRP) 3 and the downregulation of SFRP4 during osteoinduction. Functional studies showed that SFRP3 promoted and SFRP4 suppressed the osteoblastic differentiation of hMSCs. In addition, SFRP3 inhibited non-canonical WNT signalling by binding WNT5A, which is a representative non-canonical WNT protein. These results indicate the involvement of the WNT signalling pathway during the osteoblastic differentiation of hMSCs. SFRPs oppositely control osteoblastogenesis through canonical and non-canonical pathways and may be useful for directing the lineage of hMSCs in cytotherapeutic use. |
| DOI | 10.1016/j.biomaterials.2013.01.066 |
| PMID | 23384792 |