オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Human cardiac progenitor cell grafts as unrestricted source of supernumerary cardiac cells in healthy murine hearts.
掲載誌名	正式名：Stem cells (Dayton, Ohio) 略　 称：Stem Cells ISSNコード：(1549-4918)1066-5099(Linking)
掲載区分	国外
巻・号・頁	29(12),pp.2051-2061
著者・共著者	Forte Giancarlo, Pietronave Stefano, Nardone Giorgia, Zamperone Andrea, Magnani Eugenio, Pagliari Stefania, Pagliari Francesca, Giacinti Cristina, Nicoletti Carmine, Musaró Antonio, Rinaldi Mauro, Ribezzo Marco, Comoglio Chiara, Traversa Enrico, Okano Teruo, Minieri Marilena, Prat Maria, Di Nardo Paolo
発行年月	2011/12
概要	Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy.
DOI	10.1002/stem.763
PMID	22009661