ヤマト　マサユキ   YAMATO Masayuki   大和　雅之    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	CD61 enriches long-term repopulating hematopoietic stem cells.
掲載誌名	正式名：Biochemical and biophysical research communications 略　 称：Biochem Biophys Res Commun ISSNコード：(1090-2104)0006-291X(Linking)
掲載区分	国外
出版社	Elsevier
巻・号・頁	365(1),pp.176-182
著者・共著者	Umemoto Terumasa†, Yamato Masayuki, Shiratsuchi Yoshiko, Terasawa Masao, Yang Joseph, Nishida Kohji, Kobayashi Yoshiro, Okano Teruo*
担当区分	2nd著者
発行年月	2008/01
概要	Among the subsets that define hematopoietic stem cells (HSCs), CD34- c-kit+ Sca-1+ lineage marker- (CD34-KSL) cells are regarded as one of the populations that have the highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61 (integrin beta3) within the CD34-KSL population. Approximately 60% of CD34-KSL cells showed high expression of CD61. CD61HighCD34-KSL populations also exhibited significantly greater properties of HSC, such as expression of HSC markers, the side population (SP) phenotype, and ability for long-term repopulation. In both SP cells and non-SP (NSP) cells, CD61HighCD34-KSL cells also contained significantly more LTR-HSCs than CD61Low/-CD34-KSL cells. Our results indicate that CD61 is exploitable for HSC enrichment as a supportive positive cell surface marker.
DOI	10.1016/j.bbrc.2007.10.168
PMID	17983596