ナカヤマ　ヒサコ   Hisako Nakayama   中山　寿子    所属   医学部 医学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	CAPS1 RNA Editing Promotes Dense Core Vesicle Exocytosis.
掲載誌名	正式名：Cell Reports 略　 称：Cell Rep ISSNコード：22111247
掲載区分	国外
巻・号・頁	17(8),pp.2004-2014
著者・共著者	MIYAKE Kotaro†, OHTA Toshio, NAKAYAMA Hisako, DOE Nobutaka, TERAO Yuri, OIKI Eiji, NAGAMOTO Izumi, YAMASHITA Yui, ABE Takaya, NISHIKURA Kazuko, KUMANOGOH Atsushi, HASHIMOTO Kouichi, KAWAHARA Yukio*
発行年月	2016/12
概要	Calcium-dependent activator protein for secretion 1 (CAPS1) plays a distinct role in the priming step of dense core vesicle (DCV) exocytosis. CAPS1 pre-mRNA is known to undergo adenosine-to-inosine RNA editing in its coding region, which results in a glutamate-to-glycine conversion at a site in its C-terminal region. However, the physiological significance of CAPS1 RNA editing remains elusive. Here, we created mutant mice in which edited CAPS1 was solely expressed. These mice were lean due to increased energy expenditure caused by physical hyperactivity. Electrophysiological and biochemical analyses demonstrated that the exocytosis of DCVs was upregulated in the chromaffin cells and neurons of these mice. Furthermore, we showed that edited CAPS1 bound preferentially to the activated form of syntaxin-1A, a component of the exocytotic fusion complex. These findings suggest that RNA editing regulates DCV exocytosis in vivo, affecting physical activity.
DOI	10.1016/j.celrep.2016.10.073
PMID	27851964