ヨシダ　ケイタ   Yoshida Keita   吉田　慶太    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis.
掲載誌名	正式名：Development, growth&differentiation 略　 称：Dev Growth Differ ISSNコード：1440-169X(Electronic)0012-1592(Linking)
掲載区分	国外
巻・号・頁	56(7),pp.499-510
著者・共著者	Sasaki Haruka†, Yoshida Keita, Hozumi Akiko, Sasakura Yasunori*
担当区分	2nd著者
発行年月	2014/09
概要	Knockout of genes with CRISPR/Cas9 is a newly emerged approach to investigate functions of genes in various organisms. We demonstrate that CRISPR/Cas9 can mutate endogenous genes of the ascidian Ciona intestinalis, a splendid model for elucidating molecular mechanisms for constructing the chordate body plan. Short guide RNA (sgRNA) and Cas9 mRNA, when they are expressed in Ciona embryos by means of microinjection or electroporation of their expression vectors, introduced mutations in the target genes. The specificity of target choice by sgRNA is relatively high compared to the reports from some other organisms, and a single nucleotide mutation at the sgRNA dramatically reduced mutation efficiency at the on-target site. CRISPR/Cas9-mediated mutagenesis will be a powerful method to study gene functions in Ciona along with another genome editing approach using TALE nucleases.
DOI	10.1111/dgd.12149
文献番号	25212715