ヨシダ　ケイタ   YOSHIDA Keita   吉田　慶太    所属   医学部 医学科    職種   助教
論文種別	総説
言語種別	英語
査読の有無	査読なし
招待の有無	招待あり
表題	Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.
掲載誌名	正式名：Methods in molecular biology (Clifton, N.J.) 略　 称：Methods Mol Biol ISSNコード：10643745/19406029
掲載区分	国外
巻・号・頁	1630,pp.235-245
著者・共著者	Sasakura Yasunori, Yoshida Keita, Treen Nicholas
担当区分	2nd著者
発行年月	2017
概要	The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.
DOI	10.1007/978-1-4939-7128-2_19
文献番号	28643263