ヨシダ ケイタ
Yoshida Keita
吉田 慶太 所属 医学部 医学科 職種 助教 |
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論文種別 | 総説 |
言語種別 | 英語 |
査読の有無 | 査読なし |
招待の有無 | 招待あり |
表題 | Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease. |
掲載誌名 | 正式名:Methods in molecular biology (Clifton, N.J.) 略 称:Methods Mol Biol ISSNコード:10643745/19406029 |
掲載区分 | 国外 |
巻・号・頁 | 1630,pp.235-245 |
著者・共著者 | Sasakura Yasunori, Yoshida Keita, Treen Nicholas |
担当区分 | 2nd著者 |
発行年月 | 2017 |
概要 | The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods. |
DOI | 10.1007/978-1-4939-7128-2_19 |
文献番号 | 28643263 |