ヨシダ ケイタ   Yoshida Keita
  吉田 慶太
   所属   医学部 医学科
   職種   助教
論文種別 総説
言語種別 英語
査読の有無 査読なし
招待の有無 招待あり
表題 Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.
掲載誌名 正式名:Methods in molecular biology (Clifton, N.J.)
略  称:Methods Mol Biol
ISSNコード:10643745/19406029
掲載区分国外
巻・号・頁 1630,pp.235-245
著者・共著者 Sasakura Yasunori, Yoshida Keita, Treen Nicholas
担当区分 2nd著者
発行年月 2017
概要 The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.
DOI 10.1007/978-1-4939-7128-2_19
文献番号 28643263