シミズ　タツヤ   Shimizu Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cell sheet engineering for myocardial tissue reconstruction.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：(0142-9612)0142-9612(Linking)
掲載区分	国外
巻・号・頁	24(13),pp.2309-16
著者・共著者	Shimizu Tatsuya†, Yamato Masayuki, Kikuchi Akihiko, Okano Teruo*
担当区分	筆頭著者
発行年月	2003/06
概要	Myocardial tissue engineering has now emerged as one of the most promising treatments for the patients suffering from severe heart failure. Tissue engineering has currently been based on the technology using three-dimensional (3-D) biodegradable scaffolds as alternatives for extracellular matrix. According to this most popular technique, several types of 3-D myocardial tissues have been successfully engineered by seeding cardiomyocytes into poly(glycolic acid), gelatin, alginate or collagen scaffolds. However, insufficient cell migration into the scaffolds and inflammatory reaction due to scaffold biodegradation remain problems to be solved. In contrast to these technologies, we now propose novel tissue engineering methodology layering cell sheets to construct 3-D functional tissues without any artificial scaffolds. Confluent cells on temperature-responsive culture surfaces can be harvested as a viable contiguous cell sheet only by lowering temperature without any enzymatic digestions. Electrical communications are established between layered cardiomyocyte sheets, resulting in simultaneous beating 3-D myocardial tissues. Layered cardiomyocyte sheets in vivo present long survival, macroscopic pulsation and characteristic structures of native heart tissue. Cell sheet engineering should have enormous potential for fabricating clinically applicable myocardial tissues and should promote tissue engineering research fields.
DOI	https://doi.org/10.1016/S0142-9612(03)00110-8
PMID	12699668