シミズ　タツヤ   Shimizu Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Enhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model.
掲載誌名	正式名：Scientific reports 略　 称：Sci Rep ISSNコード：(2045-2322)2045-2322(Linking)
掲載区分	国外
巻・号・頁	7(1),pp.8824
著者・共著者	KAWAMURA Masashi†, MIYAGAWA Shigeru, FUKUSHIMA Satsuki, SAITO Atsuhiro, MIKI Kenji, FUNAKOSHI Shunsuke, YOSHIDA Yoshinori, YAMANAKA Shinya, SHIMIZU Tatsuya, OKANO Teruo, DAIMON Takashi, TODA Koichi, SAWA Yoshiki*
発行年月	2017/08
概要	Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted cell-sheet is a concern related to the effectiveness and durability of the treatment. Herein, we hypothesized that the combined the omentum flap might enhance survival and the therapeutic effects of hiPS-CM cell-sheet transplant for ICM treatment. Treatment by Wnt signaling molecules in hiPS cells produced hiPS-CMs, which were magnetically labeled by superparamagnetic iron oxide (SPIO), followed by culture in the thermoresponsive dishes to generate hiPS-CMs cell-sheets. A porcine ICM model included 4 groups; sham operation, omentum flap only, cell-sheet only, or combination therapy. Ejection fraction (EF) was significantly greater in the cell-sheet only and combination group compared to the other groups during the follow-up period. At 3 months, the EF of the combination group was significantly greater than that of the cell-sheet only group. Consistently, the survival rate of the SPIO-labeled hiPS-CMs, as assessed by MRI, was significantly greater in the combination group than in the cell-sheet only group. This cell delivery system would be useful in optimizing the hiPS-CM cell-sheet transplant for treating severe heart failure.
DOI	10.1038/s41598-017-08869-z
PMID	28821761