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ハラグチ ユウジ
Haraguchi Yuuji
原口 裕次 所属 医学研究科 医学研究科 (医学部医学科をご参照ください) 職種 特任准教授 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Real-time quantitation of internal metabolic activity of three-dimensional engineered tissues using an oxygen microelectrode and optical coherence tomography. |
| 掲載誌名 | 正式名：Journal of biomedical materials research. Part B, Applied biomaterials 略 称：J Biomed Mater Res B Appl Biomater ISSNコード：(1552-4981)1552-4973(Linking) |
| 掲載区分 | 国外 |
| 巻・号・頁 | 105(4),pp.855-864 |
| 著者・共著者 | Kagawa Yuki†*, Haraguchi Yuji, Tsuneda Satoshi, Shimizu Tatsuya |
| 担当区分 | 2nd著者 |
| 発行年月 | 2017/05 |
| 概要 | Recent progress in tissue engineering technology has enabled us to develop thick tissue constructs that can then be transplanted in regenerative therapies. In clinical situations, it is vital that the engineered tissues to be implanted are safe and functional before use. However, there is currently a limited number of studies on real-time quality evaluation of thick living tissue constructs. Here we developed a system for quantifying the internal activities of engineered tissues, from which we can evaluate its quality in real-time. The evaluation was achieved by measuring oxygen concentration profiles made along the vertical axis and the thickness of the tissues estimated from cross-sectional images obtained noninvasively by an optical coherence tomography system. Using our novel system, we obtained (i) oxygen concentration just above the tissues, (ii) gradient of oxygen along vertical axis formed above the tissues within culture medium, and (iii) gradient of oxygen formed within the tissues in real-time. Investigating whether these three parameters could be used to evaluate engineered tissues during culturing, we found that only the third parameter was a good candidate. This implies that the activity of living engineered tissues can be monitored in real-time by measuring the oxygen gradient within the tissues. The proposed measuring strategy can be applied to developing more efficient culturing methods to support the fabrication of engineered thick tissues, as well as providing methods to confirm the quality in real-time. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 855-864, 2017. |
| DOI | 10.1002/jbm.b.33582 |
| PMID | 26821598 |