シミズ　タツヤ   Shimizu Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section.
掲載誌名	正式名：Journal of biomedical materials research. Part B, Applied biomaterials 略　 称：J Biomed Mater Res B Appl Biomater ISSNコード：(1552-4981)1552-4973(Linking)
掲載区分	国外
巻・号・頁	105(3),pp.481-488
著者・共著者	Hasegawa Akiyuki†, Haraguchi Yuji, Oikaze Hirotoshi, Kabetani Yasuhiro, Sakaguchi Katsuhisa, Shimizu Tatsuya*
担当区分	最終著者,責任著者
発行年月	2017/04
概要	Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10-20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in detail. Additionally, the 3-D migration of cells in the bioengineered tissue was also detected using OCM and metal-labeled cells. Bovine aortic endothelial cells, but not NIH3T3 murine embryonic skin fibroblasts, actively migrated within the 3-D tissues. This study showed that the OCM system would be a valuable tool in the fields of cell biology, tissue engineering, and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 481-488, 2017.
DOI	10.1002/jbm.b.33566
PMID	26545952