シミズ　タツヤ   Shimizu Tatsuya   清水　達也    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets.
掲載誌名	正式名：Macromolecular bioscience 略　 称：Macromol Biosci ISSNコード：(1616-5195)1616-5187(Linking)
掲載区分	国外
出版社	Wiley
巻・号・頁	17(8),pp.1700073
著者・共著者	Nagase Kenichi†, Nagumo Yuhei, Kim Miri, Kim Hee-Jung, Kyung Hei-Won, Chung Hye-Jin, Sekine Hidekazu, Shimizu Tatsuya, Kanazawa Hideko, Okano Teruo, Lee Seung-Jin, Yamato Masayuki*
発行年月	2017/05
概要	Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.
DOI	10.1002/mabi.201700073
PMID	28547766