イシイ　ヤスオ   Ishii Yasuo   石井　泰雄    所属   医学部 医学科    職種   講師
論文種別	総説
言語種別	英語
査読の有無	査読あり
表題	Somatic transgenesis in the avian model system.
掲載誌名	正式名：Birth defects research. Part C, Embryo today : reviews 略　 称：Birth Defects Res C Embryo Today ISSNコード：(1542-975X)1542-975X(Linking)
掲載区分	国外
巻・号・頁	75(1),pp.19-27
著者・共著者	Ishii Yasuo, Mikawa Takashi
担当区分	筆頭著者
発行年月	2005/03
概要	The chick embryo is a versatile model system, in which classical embryology can be combined with modern molecular approaches. In the last two decades, several efficient methods have been developed to introduce exogenous genes into the chick embryo. These techniques allow alteration of gene expression levels in a spatially and temporally restricted manner, thereby circumventing embryonic lethality and/or eliminating secondary effects in other tissues. Here, we present the current status of avian somatic transgenic techniques, focusing on electroporation and retrovirus-mediated gene transfer. Electroporation allows quick and efficient gain-of-function studies based on transient misexpression of genes. Retroviral vectors, which are capable of integrating exogenous genes into the host chromosome, permit analysis of long-term effects of gene misexpression. The variety of methods available for somatic transgenesis, along with the recent completion of the chicken genome, are transforming the chick embryo into one of the most attractive model systems to examine function of genes that are important for embryonic development.
DOI	10.1002/bdrc.20033
PMID	15838916