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イシイ ヤスオ
Ishii Yasuo
石井 泰雄 所属 医学部 医学科 職種 講師 |
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| 論文種別 | 総説 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Somatic transgenesis using retroviral vectors in the chicken embryo. |
| 掲載誌名 | 正式名：Developmental dynamics : an official publication of the American Association of Anatomists 略 称：Dev Dyn ISSNコード：(1058-8388)1058-8388(Linking) |
| 掲載区分 | 国外 |
| 巻・号・頁 | 229(3),pp.630-42 |
| 著者・共著者 | Ishii Yasuo, Reese David E, Mikawa Takashi |
| 担当区分 | 筆頭著者 |
| 発行年月 | 2004/03 |
| 概要 | The avian embryo is an excellent model system for experimental studies because of its accessibility and ease of microsurgical manipulations. While the complete chicken genome sequence will soon be determined, a comprehensive germ cell transmission-based genetic approach is not available for this animal model. Several techniques of somatic cell transgenesis have been developed in the past decade. Of these, the retroviral shuttle vector system provides both (1) stable integration of exogenous genes into the host cell genome, and (2) constant expression levels in a target cell population over the course of development. This review summarizes retroviral vectors available for the avian model and outlines the uses of retroviral-mediated gene transfer for cell lineage analysis as well as functional studies of genes and proteins in the chick embryo. |
| DOI | 10.1002/dvdy.10484 |
| PMID | 14991718 |