オオノ　ヒデキ   Oono Hideki   大野　秀樹    所属   医学部 医学科（附属足立医療センター）    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains
掲載誌名	正式名：Oncogene ISSNコード：0950-9232 (Print) 0950-9232 (Linking)
掲載区分	国外
巻・号・頁	22,pp.8422-31
著者・共著者	Ohno, H., Hirabayashi, S., Kansaku, A., Yao, I., Tajima, M., Nishimura, W., Ohnishi, H., Mashima, H., Fujita, T., Omata, M., Hata, Y.
担当区分	筆頭著者
発行年月	2003
概要	MAGI-1 and CASK are membrane-associated guanylate kinases of epithelial junctions. MAGI-1 is localized at tight junctions in polarized epithelial cells, whereas CASK is localized along the lateral membranes. We obtained the KIAA0769 gene product through the yeast two-hybrid screening using MAGI-1 as a bait and named it Carom. Carom has a coiled-coil domain in the middle region, and two src homology 3 domains and a PSD-95/Dlg-A/ZO-1 (PDZ)-binding motif in the C-terminal region. Carom binds to the fifth PDZ domain of MAGI-1 and the calmodulin kinase domain of CASK in vitro. MAGI-1 and CASK bind to the distinct sequences in the C-terminal region of Carom, but still compete with each other for Carom binding. The study using a stable transformant of Madine Darby canine kidney (MDCK) cells expressing GFP-Carom revealed that Carom was partially overlapped by MAGI-1 in MDCK cells, which have not yet established mature cell junctions, but became separated from MAGI-1 and colocalized with CASK in polarized cells. Carom was highly resistant to Triton X-100 extractions and recruited CASK to the Triton X-100-insoluble structures. Carom is a binding partner of CASK, which interacts with CASK in polarized epithelial cells and may link it to the cytoskeleton.
DOI	10.1038/sj.onc.1206996
文献番号	14627983