ヤマト　マサユキ   Yamato Masayuki   大和　雅之    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：(1878-5905)0142-9612(Linking)
掲載区分	国外
巻・号・頁	34(36),pp.9010-9017
著者・共著者	KOBAYASHI Toyoshige†, KAN Kazutoshi, NISHIDA Kohji, YAMATO Masayuki, OKANO Teruo*
発行年月	2013/12
概要	We have performed clinical applications of cell sheet-based regenerative medicine with human patients in several fields. In order to achieve the mass production of transplantable cell sheets, we have developed automated cell culture systems. Here, we report an automated robotic system utilizing a cell culture vessel, cell cartridge. The cell cartridge had two rooms for epithelial cells and feeder layer cells separating by porous membrane on which a temperature-responsive polymer was covalently immobilized. After pouring cells into this robotic system, cell seeding, medium change, and microscopic examination during culture were automatically performed according to the computer program. Transplantable corneal epithelial cell sheets were successfully fabricated in cell cartridges with this robotic system. Then, fabricated cell sheets were transplanted onto ocular surfaces of rabbit limbal epithelial stem cell deficiency model after 6-h transportation using a portable homothermal container to keep inner temperature at 36 °C. Within one week after transplantation, normal corneal epithelium was successfully regenerated. This automatic cell culture system would be useful for industrialization of tissue-engineered products for regenerative medicine.
DOI	10.1016/j.biomaterials.2013.07.065
PMID	23978517