ヤマト　マサユキ   Yamato Masayuki   大和　雅之    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Peritoneal cell sheets composed of mesothelial cells and fibroblasts prevent intra-abdominal adhesion formation in a rat model.
掲載誌名	正式名：Journal of tissue engineering and regenerative medicine 略　 称：J Tissue Eng Regen Med ISSNコード：(1932-7005)1932-6254(Linking)
掲載区分	国外
出版社	Wiley Online Library
巻・号・頁	10(10),pp.855-866
著者・共著者	Kawanishi Kunio†, Yamato Masayuki, Sakiyama Ryouichi, Okano Teruo*, Nitta Kosaku
担当区分	2nd著者
発行年月	2013/12
概要	Postoperative intra-abdominal adhesions remain an unsolved problem despite significant progress in the surgical procedures themselves. They often lead to small-bowel obstruction, chronic abdominal and pelvic pain, as well as female infertility. The loss of mesothelial cells and several components of the inflammatory system following injury to the peritoneum results in fibrin formation and angiogenesis. The remaining fibrin matrix and angiogenesis lead to replacement by fibroblasts and fibrous band formation. The aim of this study was to develop a new therapeutic method of preventing intra-abdominal adhesions. We fabricated transplantable peritoneal cell sheets from the rat peritoneum by cell sheet engineering using a temperature-responsive culture system. The peritoneal cell sheets developed were composed of an upper monolayer of mesothelial cells and underlying multilayered fibroblasts, similar to the peritoneum in vivo. Transplantation of peritoneal cell sheets prevented tissue adhesion, fibrin deposition and angiogenesis, and, moreover, lymphangiogenesis and macrophage infiltration in a rat caecum cauterization adhesion model. Copyright © 2013 John Wiley & Sons, Ltd.
DOI	10.1002/term.1860
PMID	24323675