タカギ　リヨウ   Takagi Riyou   高木　亮    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：(1878-5905)0142-9612(Linking)
掲載区分	国外
出版社	Elsevier
巻・号・頁	42,pp.87-93
著者・共著者	YAMAMOTO Kazuhisa†, HAMA Takanori, YAMATO Masayuki, UCHIMIZU Hirotaka, SUGIYAMA Hiroaki, TAKAGI Ryo, YAGUCHI Yuichiro, OKANO Teruo, KOJIMA Hiromi*
発行年月	2015/02
概要	Postoperative regeneration of the middle ear mucosa and pneumatization of the middle ear cavity are of great importance after middle ear surgery. This study developed a new method to transplant autologous nasal mucosal epithelial cell sheets into the damaged middle ear cavity. The aim of this study was to evaluate postoperative healing after the transplantation of the cell sheets. Rabbit nasal mucosal epithelial cell sheets were fabricated on a temperature-responsive culture dish, and transplanted into the damaged middle ear of rabbit, which was surgically created. The healing of middle ears was evaluated by histology and X-ray computed tomography after transplantation. Functional evaluation was performed by measuring the maximum middle ear total pressure reflecting a trans-mucosal gas exchange function. Two control groups were used: the normal control group and the mucosa-eliminated control group. Transplantation of cell sheets suppressed the bone hyperplasia and the narrowing of pneumatic space in the middle ear cavity compared with the mucosa-eliminated control group. The mucosal gas exchange function was also better in the cell sheet-transplanted group. Nasal mucosal epithelial cell sheet was confirmed to be useful as an effective graft material after middle ear surgery and hopefully become a novel therapy in the future.
DOI	10.1016/j.biomaterials.2014.11.037
PMID	25542796