コモイケ　ユウタ   Komoike Yuuta   蒋池　勇太    所属   医学部 医学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Adaptive changes in TEF-1 gene expression during cold acclimation in the medaka.
掲載誌名	正式名：Zoological science 略　 称：Zoolog Sci ISSNコード：0289-0003
巻・号・頁	23(10),pp.903-8
著者・共著者	Yamasaki Yukihiko, Komoike Yuta, Higashinakagawa Toru
担当区分	2nd著者
発行年月	2006/10
概要	How animals adaptively respond to a cold or hot environment has been questioned for a long time. Recently, with the aid of microarray analysis, various temperature-sensitive genes have been identified in several species. However, a definitive hypothesis regarding the mechanism of adaptation has not been proposed. In the present study, we surveyed, in medaka (Oryzias latipes), genes for which the level of expression changes depending on the surrounding temperature. A messenger RNA differential display of medaka muscle total RNA revealed one such gene encoding transcription enhancer factor-1 (TEF-1). In medaka muscle, the TEF-1 gene produces two splicing variants, TEF-1A and TEF-1B mRNAs. During cold acclimation, the mRNA level of TEF-1A decreased, whereas that of TEF-1B increased. We also found that three putative downstream genes of TEF-1, two for myosin heavy chain (MyHC) and one for troponin T (TnT), a specific group of muscle proteins, were transcribed in a temperature-dependent manner. These results suggest that the transcription of MyHC and/or TnT is regulated by TEF-1 and that these molecules participate in muscle reconstruction during temperature adaptation in fish.
DOI	10.2108/zsj.23.903
PMID	17116993